Healthcare workers are especially at risk for burnout because of increased efficiency demands, reduced operations budgets, expedited turnaround times, and the consequences associated with patient errors. They also experience a number of negative personal and organizational outcomes from burnout, all of which have the potential to negatively affect the quality of healthcare. This study explores the extent to which spiritual leadership reduces burnout among medical laboratory personnel while positively influencing organizational commitment, work unit productivity, and employee life satisfaction. Results revealed spiritual leadership exhibited both direct and mediating effects on study outcomes. Implications for theory, research, and practice are discussed.